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If a region of the coding strand of DNA reads 5 prime-ATGCCG-3 prime, what is the sequence of the corresponding template strand and mRNA?

  • A Template: 3 prime-TACGGC-5 prime; mRNA: 5 prime-AUGCCG-3 prime
  • B Template: 5 prime-TACGGC-3 prime; mRNA: 5 prime-UACGGC-3 prime
  • C Template: 3 prime-ATGCCG-5 prime; mRNA: 5 prime-ATGCCG-3 prime
  • D Template: 3 prime-TACGGC-5 prime; mRNA: 3 prime-AUGCCG-5 prime

Correct answer: A. Template: 3 prime-TACGGC-5 prime; mRNA: 5 prime-AUGCCG-3 prime

Explanation: The template strand is complementary and antiparallel to the coding strand (3 prime-TACGGC-5 prime), and mRNA has the same sequence as the coding strand except T is replaced by U, giving 5 prime-AUGCCG-3 prime.

Concept context

DNA structure, replication, transcription, translation, the genetic code, and gene regulation (lac operon). The single most important chapter for NEET molecular biology.

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