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A mutation that converts the lac operon's operator sequence so the repressor can never bind would most likely result in:

  • A The operon would become largely switched off, even when lactose is present in the cell
  • B Constitutive (continuous) expression of the z, y, and a genes regardless of lactose presence
  • C Complete and total loss of RNA polymerase's ability to bind to the operon's promoter region
  • D No change would occur, since the operator sequence does not affect transcription rates

Correct answer: B. Constitutive (continuous) expression of the z, y, and a genes regardless of lactose presence

Explanation: If the repressor cannot bind the mutated operator, it can no longer block RNA polymerase, so the structural genes would be transcribed constitutively, irrespective of whether lactose (inducer) is present.

Concept context

DNA structure, replication, transcription, translation, the genetic code, and gene regulation (lac operon). The single most important chapter for NEET molecular biology.

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