- A Charge only
- B Size (molecular weight)
- C Isoelectric point
- D Amino acid composition
Correct answer: B. Size (molecular weight)
Explanation: SDS-PAGE: SDS denatures proteins and gives them uniform negative charge proportional to size. Proteins separate by size in polyacrylamide gel.
Concept context
Cell theory, prokaryotic vs eukaryotic cells, organelles and their functions. Foundation of all biology.