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The role of 14-3-3 proteins in stomatal regulation is:

  • A Forming the selective pore of the inward-rectifying K+ channel itself directly within the guard cell plasma membrane
  • B Acting as signaling scaffolds that activate H+ ATPase when bound to its phosphorylated C-terminus, promoting stomatal opening
  • C Hydrolyzing abscisic acid molecules directly into inactive breakdown products within the surrounding guard cell cytosol
  • D Synthesizing entirely new K+ channel protein subunits specifically during the active stomatal opening response phase

Correct answer: B. Acting as signaling scaffolds that activate H+ ATPase when bound to its phosphorylated C-terminus, promoting stomatal opening

Explanation: 14-3-3 proteins bind phosphorylated H+ ATPase C-terminus (phosphorylated by protein kinase in response to light/fusicoccin), activating the pump. H+ efflux hyperpolarizes membrane, driving K+ influx and stomatal opening.

Concept context

Water and mineral absorption, the ascent of sap, transpiration, and phloem transport via the pressure flow hypothesis.

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