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The polymerase chain reaction (PCR) requires which temperature to denature DNA?

  • A 37 C
  • B 55 C
  • C 72 C
  • D 94-95 C

Correct answer: D. 94-95 C

Explanation: PCR denaturation step: DNA is heated to ~94-95 degrees C to separate double-stranded DNA into single strands.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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