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The Cas9 protein in CRISPR is guided to its target by:

  • A Protein-protein interactions with transcription factor complexes
  • B Guide RNA (gRNA) complementary to the target DNA sequence
  • C Restriction enzymes that recognize specific palindromic sequences
  • D Diffusible chemical signals released by the target cell

Correct answer: B. Guide RNA (gRNA) complementary to the target DNA sequence

Explanation: CRISPR-Cas9: a guide RNA (gRNA) is designed to match the target DNA sequence. It directs the Cas9 nuclease to cut at that specific location.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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