- A Blunt cuts straight across both DNA strands
- B Staggered cuts leaving single-stranded overhangs
- C Circular cuts that excise a closed DNA loop
- D Random cuts at unpredictable genomic positions
Correct answer: B. Staggered cuts leaving single-stranded overhangs
Explanation: Staggered cuts by restriction enzymes leave short single-stranded extensions (sticky/cohesive ends) that can pair with complementary sticky ends.
Concept context
Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.
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