- A Calculating a single averaged gene expression value across an entire bulk tissue sample collected
- B Gene expression profiling of individual cells, revealing cellular heterogeneity and cell types within tissues
- C Analysis that is restricted mainly to bulk tissue homogenates, and rarely individual single cells directly
- D Profiling restricted mainly to overall protein expression levels, rather than mRNA transcript abundance
Correct answer: B. Gene expression profiling of individual cells, revealing cellular heterogeneity and cell types within tissues
Explanation: scRNA-seq: RNA from individual cells is captured, barcoded, and sequenced. Reveals transcriptome of each cell. Identifies rare cell types, transitions, trajectories, and heterogeneity invisible in bulk sequencing.
Concept context
Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.
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