- A Two largely different names that generally describe the exact same underlying phenomenon observed during normal conditions
- B The balance between desired gene editing at the intended site vs. unintended cuts at similar sequences elsewhere in the genome
- C A measure that reflects mainly the physical size of the double-strand break that was created nearby as generally observed
- D A measure that reflects mainly the total length of the guide RNA used in the experiment conducted in typical laboratory settings
Correct answer: B. The balance between desired gene editing at the intended site vs. unintended cuts at similar sequences elsewhere in the genome
Explanation: CRISPR specificity: on-target = desired edit at correct genomic location. Off-target = unintended cuts at sequences with partial complementarity to gRNA. Reduced by high-fidelity Cas9 variants, paired nickases, or base editors.
Concept context
Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.
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