- A It is reported to be limited mainly to sequencing very short DNA reads under fifty base pairs in length generally according to most researchers
- B It sequences millions of DNA fragments simultaneously in parallel (massively parallel sequencing), dramatically reducing cost and time
- C It is said to largely reduce the need for any PCR amplification step to be performed beforehand on the sample in the majority of cases studied
- D It is reported to be capable of sequencing mainly RNA molecules directly, and is said to rarely sequence genomic DNA as widely reported
Correct answer: B. It sequences millions of DNA fragments simultaneously in parallel (massively parallel sequencing), dramatically reducing cost and time
Explanation: NGS (Illumina, PacBio, Nanopore): millions of DNA fragments sequenced simultaneously. Much faster and cheaper than Sanger. Used for whole genome sequencing, RNA-seq, ChIP-seq, etc.
Concept context
Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.
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