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In forensic DNA profiling, which technique is used to analyze STR loci?

  • A Southern blot hybridization using a radioactive DNA probe
  • B PCR amplification followed by capillary electrophoresis
  • C Northern blot hybridization to detect specific mRNA transcripts
  • D Western blot detection using a labeled antibody probe

Correct answer: B. PCR amplification followed by capillary electrophoresis

Explanation: Forensic DNA profiling: PCR amplifies multiple STR (short tandem repeat) loci. Fragment sizes are measured by capillary electrophoresis. Results create a unique DNA profile.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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