- A Point mutations scattered randomly across the entire genome of a particular cell line studied according to conventional understanding
- B Genome-wide protein-DNA interactions and histone modifications (where transcription factors bind, which regions are active)
- C Overall mRNA expression patterns averaged uniformly across an entire bulk tissue sample collected in routine practice
- D Mainly the precise positions of methylated cytosine bases located within CpG island regions nearby overall in most cases
Correct answer: B. Genome-wide protein-DNA interactions and histone modifications (where transcription factors bind, which regions are active)
Explanation: ChIP-seq: protein cross-linked to DNA, chromatin sheared, antibody pulls down protein-DNA complexes. DNA sequenced to map genome-wide binding sites of transcription factors, histones, etc.
Concept context
Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.
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