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ATAC-seq reveals:

  • A Quantitative mRNA expression levels that are generally averaged uniformly across an entire population of cells studied closely under usual circumstances
  • B Genome-wide open chromatin regions (accessible, active regulatory elements) by transposase-accessible chromatin with high-throughput sequencing
  • C Mainly the overall pattern of cytosine methylation occurring across the entire genome of the cell being studied closely according to most researchers
  • D Mainly the precise genomic location of repetitive, transposon-derived DNA sequences scattered within the chromatin region in the majority of cases studied

Correct answer: B. Genome-wide open chromatin regions (accessible, active regulatory elements) by transposase-accessible chromatin with high-throughput sequencing

Explanation: ATAC-seq: Tn5 transposase cuts and tags accessible (open) chromatin. Sequencing maps genome-wide open chromatin regions, identifying active promoters, enhancers, and regulatory elements.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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