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In making nematode-resistant tobacco by RNA interference, gene silencing is triggered by:

  • A single-stranded DNA
  • B a plasmid vector
  • C a restriction enzyme
  • D double-stranded RNA

Correct answer: D. double-stranded RNA

Explanation: RNAi is triggered by double-stranded RNA, which silences the complementary nematode mRNA and stops the parasite.

Concept context

GM crops, transgenic animals, gene therapy, molecular diagnostics, and bioethics. Frequent 2–3 mark questions in NEET.

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