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Which enzyme catalyzes the rate-limiting step of glycolysis?

  • A Aldolase
  • B Phosphoglucose isomerase
  • C Phosphofructokinase-1
  • D Pyruvate kinase

Correct answer: C. Phosphofructokinase-1

Explanation: Phosphofructokinase-1 (PFK-1) catalyzes the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate. It is the key regulatory enzyme of glycolysis, inhibited by ATP and citrate.

Concept context

Carbohydrates, proteins, lipids, nucleic acids, and enzyme kinetics. Essential foundation for understanding metabolism.

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