- A Type I cuts within the recognition site; Type II cuts elsewhere
- B Type II cuts within or near recognition site; Type I cuts away from it
- C Type I requires ATP; Type II mainly requires Mg2+ instead
- D Type II works mainly on RNA substrates specifically
Correct answer: B. Type II cuts within or near recognition site; Type I cuts away from it
Explanation: Type II restriction enzymes (used in genetic engineering) cut within or very close to their recognition sequence, producing predictable fragments. Type I enzymes cut far from their recognition site, making them impractical for cloning.
Concept context
Carbohydrates, proteins, lipids, nucleic acids, and enzyme kinetics. Essential foundation for understanding metabolism.