- A The maximum reaction rate achieved when the enzyme is fully saturated
- B Substrate concentration at half Vmax
- C The total enzyme concentration present within the reaction mixture
- D The pH value at which the enzyme shows peak catalytic activity
Correct answer: B. Substrate concentration at half Vmax
Explanation: Km is the substrate concentration at which the reaction velocity is half of Vmax. A lower Km means higher affinity of enzyme for substrate.
Concept context
Carbohydrates, proteins, lipids, nucleic acids, and enzyme kinetics. Essential foundation for understanding metabolism.