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A non-competitive inhibitor affects enzyme kinetics by

  • A Increasing Km and decreasing Vmax
  • B Decreasing Vmax without changing Km
  • C Increasing Vmax without changing Km
  • D Decreasing Km only

Correct answer: B. Decreasing Vmax without changing Km

Explanation: Non-competitive inhibitors bind to the allosteric site (not active site) and reduce Vmax by decreasing enzyme efficiency. Km is unchanged because substrate can still bind with same affinity.

Concept context

Carbohydrates, proteins, lipids, nucleic acids, and enzyme kinetics. Essential foundation for understanding metabolism.

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